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Goat anti-MBD2 (isoform 1) Antibody
Item#:
dAP-0986
Size Price Quantity
50.0 ug $310.00
Goat anti-MBD2 (isoform 1) Antibody
Principle Name MBD2 (isoform 1)
Official Symbol MBD2
All Name and Symbols MBD2; methyl-CpG binding domain protein 2 ; DKFZp586O0821; DMTase; NY-CO-41
Accession Number(s) NP_003918.1
Peptide Sequence RNDPLNQNKGKPDLN, is from internal region
Human Gene ID(s) 8932
Non-Human GeneID(s) 17191 (mouse);
Important Comments This antibody is expected to resognize isoform 1 (NP_003918.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio
Immunohistochemistry In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4µg/ml.
Reference(s) Auriol E, Billard LM, Magdinier F, Dante R. Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus. Nucleic Acids Res. 2005 Jul 28;33(13)

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