Goat anti-ARF1 (variants 1-4) Antibody

Item#:

dAP-0981

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ARF1 (variants 1-4) Antibody
Principle Name	ARF1 (variants 1-4)
Official Symbol	ARF1
All Name and Symbols	#Deleted
Accession Number(s)	NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1
Peptide Sequence	EGLDWLSNQLRNQK, is from C Terminus
Human Gene ID(s)	375; 376, 377, 378
Non-Human GeneID(s)	11840 (mouse); 64310 (rat);
Important Comments	This antibody is expected to recognize all four reported isoforms.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Approx 18kDa band observed in lysates of cell lines HEK293, A431 and HepG2 (calculated MW of 20.7kDa according to NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Recommended concentration
Reference(s)	Dubois T, Paleotti O, Mironov AA, Fraisier V, Stradal TE, De Matteis MA, Franco M, Chavrier P. Golgi-localized GAP for Cdc42 functions downstream of ARF1 to control Arp2/3 complex and F-actin dynamics. Nat Cell Biol. 2005 Apr;7(4)