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Goat anti-DAP10 / HCST Antibody
Item#:
dAP-0970
Size Price Quantity
50.0 ug $310.00
Goat anti-DAP10 / HCST Antibody
Principle Name DAP10 / HCST
Official Symbol HCST
All Name and Symbols HCST; DAP10; hematopoietic cell signal transducer; KAP10; PIK3AP ; DNAX-activation protein 10; phosphoinositide-3-kinase adaptor protein
Accession Number(s) NP_055081.1; NP_001007470.1
Peptide Sequence RSPAQEDGKVYIN, is from C Terminus
Human Gene ID(s) 10870
Non-Human GeneID(s) 23900 (mouse); 474146 (rat);
Important Comments This antibody is expected to recognise isoform 1 (NP_055081.1) but it may recognize isoform 2 lacking residue E81 (NP_001007470.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 40kDa band observed in human spleen lysates (calculated MW of 9.5kDa according to NP_055081.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wu et al, Science. 1999 Jul 30;28
Reference(s) Upshaw JL, Arneson LN, Schoon RA, Dick CJ, Billadeau DD, Leibson PJ. NKG2D-mediated signaling requires a DAP10-bound Grb2-Vav1 intermediate and phosphatidylinositol-3-kinase in human natural killer cells. Nat Immunol. 2006 May;7(5)

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