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Goat anti-PIK3CA / P110alpha Antibody
Item#:
dAP-0964
Size Price Quantity
50.0 ug $310.00
Goat anti-PIK3CA / P110alpha Antibody
Principle Name PIK3CA / P110alpha
Official Symbol PIK3CA
All Name and Symbols PIK3CA; p110-alpha; phosphoinositide-3-kinase, catalytic, alpha polypeptide ; MGC142161; MGC142163; PI3K; PI3-kinase p110 subunit alpha; PtdIns-3-kinase p110; phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha; phosphatidylinositol 3-kinase, catalyti
Accession Number(s) NP_006209.2
Peptide Sequence HQWLKDKNKGEIYD, is from internal region
Human Gene ID(s) 5290
Non-Human GeneID(s) 18706 (mouse); 170911 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1µg/ml).
Immunohistochemistry In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration
Reference(s) Lopez-Knowles E, Hernandez S, Malats N, Kogevinas M, Lloreta J, Carrato A, Tardon A, Serra C, Real FX. PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors. Cancer Res. 2006 Aug 1;66(15)

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