Goat anti-GFAP Antibody |
| Principle Name | GFAP |
| Official Symbol | GFAP |
| All Name and Symbols | GFAP; glial fibrillary acidic protein ; FLJ45472 ; intermediate filament protein |
| Accession Number(s) | NP_002046.1 |
| Peptide Sequence | DGEVIKESKQEHKD, is from C Terminus |
| Human Gene ID(s) | 2670 |
| Non-Human GeneID(s) | 14580 (mouse); 24387 (rat); |
| Important Comments | GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells but its exact function remains poorly understood, despite the number of studies using it as a cell marker. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 48-50kDa band observed in Human Cerebellum and Mouse and Rat Brain lysates (calculated MW of 49.9kDa according to Human NP_002046.1, Mouse NP_034407.2 and Rat NP_058705.2). Recommended concentration |
| Reference(s) | Korolainen MA, Auriola S, Nyman TA, Alafuzoff I, Pirttila T. Proteomic analysis of glial fibrillary acidic protein in Alzheimer's disease and aging brain. Neurobiol Dis. 2005 Dec;20(3) |
