Goat anti-SBP2 Antibody |
| Principle Name | SBP2 |
| Official Symbol | SECISBP2 |
| All Name and Symbols | SBP2 ; RP11-89K14.1; SECISBP2; SECIS binding protein 2 ; DKFZp686C09169; OTTHUMP00000064929; OTTHUMP00000064930; OTTHUMP00000064931; OTTHUMP00000064932; selenocysteine insertion sequence binding protein 2 |
| Accession Number(s) | NP_076982.3 |
| Peptide Sequence | KERQERKQRLQEN, is from internal region |
| Human Gene ID(s) | 79048 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IP, IHC, ICC, IF |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 120kDa band detected in HFF cell lysates. Data kindly provided by Laura Papp and Kum Kum Khanna. The observed molecular weight corresponds to earlier findings in literature with different antibodies (Papp et al, Mol Cell Biol. 2006 J |
| Immunohistochemistry | In paraffin embedded Human Testis shows granular cytoplasm staining in Leydig cells. Recommended concentration, 3µg/ml. |
| Reference(s) | Papp LV, Lu J, Striebel F, Kennedy D, Holmgren A, Khanna KK. The redox state of SECIS binding protein 2 controls its localization and selenocysteine incorporation function. Mol Cell Biol. 2006 Jul;26(13) |
