Goat anti-CPT1A Antibody |
| Principle Name | CPT1A |
| Official Symbol | CPT1A |
| All Name and Symbols | CPT1A; carnitine palmitoyltransferase 1A (liver) ; CPT1; CPT1-L; L-CPT1 ; carnitine palmitoyltransferase 1A; carnitine palmitoyltransferase I; carnitine palmitoyltransferase I, liver |
| Accession Number(s) | NP_001867.2; NP_001027017.1 |
| Peptide Sequence | DPAQTVEQRLKLFK, is from internal region |
| Human Gene ID(s) | 1374 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001867.2; NP_001027017.1) but is not expected to recognise CPT1B. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 80kDa band observed in Human Liver lysates (calculated MW of 86.2kDa according to NP_001027017.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Kidney shows mitochindrial staining in PCT. Recommended concentration |
| Reference(s) | Pocai A, Lam TK, Obici S, Gutierrez-Juarez R, Muse ED, Arduini A, Rossetti L. Restoration of hypothalamic lipid sensing normalizes energy and glucose homeostasis in overfed rats. J Clin Invest. 2006 Apr;116(4) |
