Goat anti-XRCC4-like factor / NHEJ1 Antibody

Item#:

dAP-0948

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-XRCC4-like factor / NHEJ1 Antibody
Principle Name	XRCC4-like factor / NHEJ1
Official Symbol	NHEJ1
All Name and Symbols	NHEJ1; XRCC4-like factor; nonhomologous end-joining factor 1; FLJ12610; XLF ; Cernunnos
Accession Number(s)	NP_079058.1
Peptide Sequence	QRPQLSKVKRKKPR, is from C Terminus
Human Gene ID(s)	79840
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band w
Immunohistochemistry	In paraffin embedded Human Placenta shows nuclear staining in trophoblasts. Recommended concentration, 2.5-3.8µg/ml.
Reference(s)	Ahnesorg P, Smith P, Jackson SP. XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA nonhomologous end-joining. Cell. 2006 Jan 27;124(2)