Goat anti-CHA / TCFL5 Antibody

Item#:

dAP-0946

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CHA / TCFL5 Antibody
Principle Name	CHA / TCFL5
Official Symbol	TCFL5
All Name and Symbols	TCFL5; CHA; transcription factor-like 5 (basic helix-loop-helix); E2BP-1; Figlb; MGC46135 ; HPV-16 E2 binding protein 1; transcription factor-like 5 protein; Cha transcription factor
Accession Number(s)	NP_006593.2
Peptide Sequence	KYIQERHGDSLKKE, is from internal region
Human Gene ID(s)	10732
Non-Human GeneID(s)	277353 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 55kDa band observed in Human Testis lysates (calculated MW of 52.7kDa according to NP_006593.2). Recommended concentration
Reference(s)	Siep M, Sleddens-Linkels E, Mulders S, van Eenennaam H, Wassenaar E, Van Cappellen WA, Hoogerbrugge J, Grootegoed JA, Baarends WM. Basic helix-loop-helix transcription factor Tcfl5 interacts with the Calmegin gene promoter in mouse spermatogenesis. Nucleic Acids Res. 2004 Dec 7;32(21)