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Goat anti-PEBP1 / RKIP (Internal) Antibody


Item#:
dAP-0943
Size Price Quantity
50.0 ug $310.00
Goat anti-PEBP1 / RKIP (Internal) Antibody
Principle Name PEBP1 / RKIP (Internal)
Official Symbol PEBP1
All Name and Symbols PEBP1; RKIP; phosphatidylethanolamine binding protein 1; HCNP; PBP; PEBP; prostatic binding protein; RAF kinase inhibitor protein
Accession Number(s) NP_002558.1
Peptide Sequence DPDAPSRKDPKYRE, is from internal region
Human Gene ID(s) 5037
Non-Human GeneID(s) 23980 (mouse); 29542 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 21kDa band observed in Human Prostate lysates (calculated MW of 21.1kDa according to NP_002558.1). Recommended concentration
Immunohistochemistry In paraffin embedded Adrenal Gland shows strong stinaing of secretory cells. Recommended concentration, 2-5µg/ml.
Reference(s) Yeung K, Seitz T, Li S, Janosch P, McFerran B, Kaiser C, Fee F, Katsanakis KD, Rose DW, Mischak H, Sedivy JM, Kolch W. Suppression of Raf-1 kinase activity and MAP kinase signalling by RKIP. Nature. 1999 Sep 9;401(6749)

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