Goat anti-Lipin 3 Antibody

Item#:

dAP-0940

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Lipin 3 Antibody
Principle Name	Lipin 3
Official Symbol	LPIN3
All Name and Symbols	LPIN3; lipin 3 ; RP4-620E11.3; LIPN3L; SMP2; dJ450M14.2; dJ450M14.3; dJ620E11.2 ; lipin 3-like
Accession Number(s)	NP_075047.1
Peptide Sequence	KPKQKEDAVATD, is from internal region
Human Gene ID(s)	64900
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Preliminary experiments gave bands at approx 80kDa and 60kDa in Human Liver lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated s
Immunohistochemistry	In paraffin embedded Human Thyroid Gland shows staining of cytoplasm and cell membrane of epithelial cells surrounding the follicles. Recommended concentration, 2-3µg/ml.
Reference(s)	Santos-Rosa H, Leung J, Grimsey N, Peak-Chew S, Siniossoglou S. The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J. 2005 Jun 1;24(11)