Goat anti-SIRT1 Antibody |
| Principle Name | SIRT1 |
| Official Symbol | SIRT1 |
| All Name and Symbols | SIRT1 ; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) ; RP11-57G10.3; SIR2L1 ; OTTHUMP00000060745; SIR2alpha; sir2-like 1; sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; sirtuin 1; sirtui |
| Accession Number(s) | NP_036370.2 |
| Peptide Sequence | EITEKPPRTQKE, is from internal region |
| Human Gene ID(s) | 23411 |
| Non-Human GeneID(s) | 93759 (mouse); 309757 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 120kDa band observed in Mouse fetal Brain lysates (calculated MW of 80.3kDa according to Muose NP_062786.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Nat |
| Reference(s) | Chen D, Steele AD, Lindquist S, Guarente L. Increase in activity during calorie restriction requires Sirt1. Science. 2005 Dec 9;310(5754) |
