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Goat anti-FLAP / ALOX5AP (internal) Antibody
Item#:
dAP-0925
Size Price Quantity
50.0 ug $310.00
Goat anti-FLAP / ALOX5AP (internal) Antibody
Principle Name FLAP / ALOX5AP (internal)
Official Symbol ALOX5AP
All Name and Symbols ALOX5AP; FLAP; arachidonate 5-lipoxygenase-activating protein; MK-886-binding protein; five-lipoxygenase activating protein
Accession Number(s) NP_001620.2; NP_001191335.1
Peptide Sequence HKVEHESRTQNGR, is from internal region
Human Gene ID(s) 241
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize both reported isoforms (NP_001620.2; NP_001191335.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 16kDa band observed in lysates of U937 and of Peripheral Blood Mononucleocytes (calculated MW of 18.2kDa according to NP_001620.2). Recommended concentration
Immunohistochemistry In paraffin embedded Human Spleen shows staining of select splenocytes. Recommended concentration, 3-6µg/ml.
Reference(s) Kaaman M, Ryden M, Axelsson T, Nordstrom E, Sicard A, Bouloumie A, Langin D, Arner P, Dahlman I. ALOX5AP expression, but not gene haplotypes, is associated with obesity and insulin resistance. Int J Obes (Lond). 2006 Mar;30(3)

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