Goat anti-GPX4 (Isoform a and c) Antibody

Item#:

dAP-0921

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GPX4 (Isoform a and c) Antibody
Principle Name	GPX4 (Isoform a and c)
Official Symbol	GPX4
All Name and Symbols	GPX4; glutathione peroxidase 4 (phospholipid hydroperoxidase) ; HGNC
Accession Number(s)	NP_002076.2; NP_001034937.1
Peptide Sequence	EEPLVIEKDLPHY, is from C Terminus
Human Gene ID(s)	2879
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 19kDa band observed in Human Kidney and Testis lysates and in lysates of cell line HEK293 (calculated MW of 22.1kDa according to NP_002076.2). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Testis shows strong staining surrounding the nuclei of spermatocytes and surrounding spermatozoa. Recommended concentration, 0.3µg/ml.
Reference(s)	Su D, Novoselov SV, Sun QA, Moustafa ME, Zhou Y, Oko R, Hatfield DL, Gladyshev VN. Mammalian selenoprotein thioredoxin/glutathione reductase