Goat anti-VHL Antibody |
| Principle Name | VHL |
| Official Symbol | VHL |
| All Name and Symbols | VHL; von Hippel-Lindau tumor suppressor; HRCA1; RCA1; VHL1 ; elongin binding protein; von Hippel-Lindau syndrome |
| Accession Number(s) | NP_000542.1; NP_937799.1 |
| Peptide Sequence | RSLVKPENYRRLD, is from internal region |
| Human Gene ID(s) | 7428 |
| Non-Human GeneID(s) | 22346 (mouse); 24874 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC, EIA |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Kidney. Recommended concentration |
| Reference(s) | Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J. Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha. Mol Cell Biol. 2006 Mar;26(6) |
