Goat anti-Wilms tumor 1 / WT1 Antibody

Item#:

dAP-0911

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Wilms tumor 1 / WT1 Antibody
Principle Name	Wilms tumor 1 / WT1
Official Symbol	WT1
All Name and Symbols	WT1; Wilms tumor 1; GUD; WAGR; WIT-2; WT33
Accession Number(s)	NP_000369.3; NP_077742.2; NP_077743.2; NP_077744.3
Peptide Sequence	QDPASTCVPEPASQH, is from N Terminus
Human Gene ID(s)	7490
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise all four reported isoforms (NP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 55kDa band observed in Human Kidney, Spleen and Testis lysates (calculated MW of 54.3kDa according to NP_000369.3). Recommended concentration
Reference(s)	Rao MK, Pham J, Imam JS, MacLean JA, Murali D, Furuta Y, Sinha-Hikim AP, Wilkinson MF. Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis. Genes Dev. 2006 Jan 15;20(2)