Goat anti-LEF1 Antibody |
| Principle Name | LEF1 |
| Official Symbol | LEF1 |
| All Name and Symbols | LEF1; lymphoid enhancer-binding factor 1 ; HGNC |
| Accession Number(s) | NP_057353.1 |
| Peptide Sequence | QHEQRKEQEPKRPH, is from internal region |
| Human Gene ID(s) | 51176 |
| Non-Human GeneID(s) | 16842 (mouse); 161452 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 50kDa band observed in MOLT4 lysates (calculated MW of 44.2kDa according to NP_057353.1). The observed molecular weight corresponds to earlier findings in literature (Yasumoto et al , EMBO J. 2002 Jun 3;21(11) |
| Reference(s) | Radtke F, Clevers H. Self-renewal and cancer of the gut |
