Goat anti-COMT (internal) Antibody

Item#:

dAP-0900

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-COMT (internal) Antibody
Principle Name	COMT (internal)
Official Symbol	COMT
All Name and Symbols	COMT; catechol-O-methyltransferase
Accession Number(s)	NP_000745.1; NP_009294.1
Peptide Sequence	QDIIPQLKKKYDVD, is from internal region
Human Gene ID(s)	1312
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_000745.1 and NP_009294.1)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, EIA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Approx 28kDa band in Human Testis lysates (calculated MW of 30.0kDa according to NP_000745.1 and 24.5kDa according to NP_009294.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Brain (Cerebellum) shows staining of processes coming from the edge of the granular zone into the molecular layer. Recommended concentration
Reference(s)	Tunbridge EM, Harrison PJ, Weinberger DR. Catechol-o-Methyltransferase, Cognition, and Psychosis