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Goat anti-Semaphorin 5A Antibody
Item#:
dAP-0886
Size Price Quantity
50.0 ug $310.00
Goat anti-Semaphorin 5A Antibody
Principle Name Semaphorin 5A
Official Symbol SEMA5A
All Name and Symbols SEMA5A; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5A; HGNC
Accession Number(s) NP_003957.1
Peptide Sequence DEATKKACYSKGKS, is from internal region
Human Gene ID(s) 9037
Non-Human GeneID(s) 20356 (mouse); 310207 (rat);
Important Comments The immunizing peptide corresponds to a part of the extracellular domain.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Preliminary experiments gave an approx 90kDa in Mouse Brain lysates and approx 25kDa band in Human Brain (Cerebellum), Colon, Heart and Kidney lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanati
Immunohistochemistry In paraffin embedded Human Kidney shows membanous and vesicular staining in PCT cells. Recommended concentration
Reference(s) Maraganore DM, de Andrade M, Lesnick TG, Strain KJ, Farrer MJ, Rocca WA, Pant PV, Frazer KA, Cox DR, Ballinger DG. High-resolution whole-genome association study of Parkinson disease Am J Hum Genet. 2005 Nov;77(5)

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