Goat anti-CILP Antibody |
| Principle Name | CILP |
| Official Symbol | CILP |
| All Name and Symbols | CILP; cartilage intermediate layer protein, nucleotide pyrophosphohydrolase ; HGNC |
| Accession Number(s) | NP_003604.2 |
| Peptide Sequence | NYRRTDHEDPRVK, is from internal region |
| Human Gene ID(s) | 8483 |
| Non-Human GeneID(s) | 214425 (mouse); 315761 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 150kDa band observed in rat spinal cord lysates (calculated MW of 133kDa according to human NP_003604.2 and of 132kDa according to Rat NP_001101631.1). Recommended concentration |
| Reference(s) | Seki S, Kawaguchi Y, Chiba K, Mikami Y, Kizawa H, Oya T, Mio F, Mori M, Miyamoto Y, Masuda I, Tsunoda T, Kamata M, Kubo T, Toyama Y, Kimura T, Nakamura Y, Ikegawa S. A functional SNP in CILP, encoding cartilage intermediate layer protein, is associated with susceptibility to lumbar disc disease. Nat Genet. 2005 Jun;37(6) |
