Goat anti-Radixin Antibody

Item#:

dAP-0882

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Radixin Antibody
Principle Name	Radixin
Official Symbol	RDX
All Name and Symbols	RDX; radixin
Accession Number(s)	NP_002897.1
Peptide Sequence	QARDETKKTQND, is from internal region
Human Gene ID(s)	5962
Non-Human GeneID(s)	19684 (mouse); 315655 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse, Rat
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Approx 80kDa band observed in Human Lymph Node, Tonsil and Spleen lysates (calculated MW of 68.6kDa according to NP_002897.1). The same 80kDa was also observed in lysates of several Mouse and Rat tissues and in lysates of cell lines NIH3T3
Immunohistochemistry	Paraffin embedded Human Placenta and Kidney. Recommended concentration
Reference(s)	Ivetic A, Florey O, Deka J, Haskard DO, Ager A, Ridley AJ. Mutagenesis of the ezrin-radixin-moesin binding domain of L-selectin tail affects shedding, microvillar positioning, and leukocyte tethering. J Biol Chem. 2004 Aug 6;279(32)