Goat anti-OCT4 / POU5F1 Antibody

Item#:

dAP-0878

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-OCT4 / POU5F1 Antibody
Principle Name	OCT4 / POU5F1
Official Symbol	POU5F1
All Name and Symbols	POU5F1; OTF4; POU domain, class 5, transcription factor 1; HGNC
Accession Number(s)	NP_002692.2; NP_976034.3
Peptide Sequence	C-VTTLGSPMHSN, is from C Terminus
Human Gene ID(s)	5460
Non-Human GeneID(s)	18999 (mouse); 294562 (rat);
Important Comments	This antibody is expected to recognise both reported isoforms (NP_002692.2 and NP_976034.3)
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Approx 30kDa band observed in Human Placenta, and approx 28kDa in Mouse Heart lysates (calculated MW of 30.1kDa according to Human NP_976034.3 and 25.0kDa according to Mouse NP_001239381.1). Recommended concentration
Reference(s)	Jones TD, Ulbright TM, Eble JN, Baldridge LA, Cheng L. OCT4 staining in testicular tumors