Goat anti-Ezrin / villin 2 Antibody

Item#:

dAP-0873

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Ezrin / villin 2 Antibody
Principle Name	Ezrin / villin 2
Official Symbol	VIL2
All Name and Symbols	villin 2 (ezrin) ; ezrin; VIL2; CVIL; CVL; DKFZp762H157; FLJ26216; MGC1584 ; cytovillin; villin 2
Accession Number(s)	NP_003370.2
Peptide Sequence	QDYEEKTKKAER, is from internal region
Human Gene ID(s)	7430
Non-Human GeneID(s)	22350 (mouse); 54319 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 80kDa band observed in Human Placenta lysates (calculated MW of 69.4kDa according to NP_003370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zhu et al, Am J Physiol Cell Ph
Immunohistochemistry	In paraffin embedded Human Small Intestine shows strong staining of enterocytes. Recommended concentration
Reference(s)	Stanasila L, Abuin L, Diviani D, Cotecchia S. Ezrin directly interacts with the alpha1b-adrenergic receptor and plays a role in receptor recycling. J Biol Chem. 2006 Feb 17;281(7)