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Goat anti-NET1 / ARHGEF8 (Internal) Antibody
Item#:
dAP-0866
Size Price Quantity
50.0 ug $310.00
Goat anti-NET1 / ARHGEF8 (Internal) Antibody
Principle Name NET1 / ARHGEF8 (Internal)
Official Symbol NET1
All Name and Symbols NET1; ARHGEF8; neuroepithelial cell transforming gene 1; NET1A; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene); neuroepithelioma transforming gene 1; p65 Net1 proto-oncogene protein; small GTP-binding prot
Accession Number(s) NP_005854.2; NP_001040625.1
Peptide Sequence DKLEYLDEKQRDPR, is from internal region
Human Gene ID(s) 10276
Non-Human GeneID(s) 56349 (mouse); 307098 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 80-90kDa double band in Human Placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated
Immunohistochemistry In paraffin embedded Human Breast shows cytoplasm staining of myoepithelial cells of ducts. Recommended concentration, 2.5µg/ml.
Reference(s) Qin H, Carr HS, Wu X, Muallem D, Tran NH, Frost JA. Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. J Biol Chem. 2005 Mar 4;280(9)

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