Goat anti-WNT3 Antibody |
| Principle Name | WNT3 |
| Official Symbol | WNT3 |
| All Name and Symbols | WNT3 ; wingless-type MMTV integration site family, member 3 ; INT4; MGC131950; MGC138321; MGC138323 ; WNT-3 proto-oncogene protein precursor |
| Accession Number(s) | NP_110380.1 |
| Peptide Sequence | CGRGHNTRTEKRKEK, is from internal region |
| Human Gene ID(s) | 7473 |
| Non-Human GeneID(s) | 22415 (mouse); 24882 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 40kDa band observed in lysates of cell line HEK293 (calculated MW of 39.6kDa according to NP_110380.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Breast shows cytoplasm staining in some luminal epithelial cells. Recommended concentration |
| Reference(s) | Schmitt AM, Shi J, Wolf AM, Lu CC, King LA, Zou Y. Wnt-Ryk signalling mediates medial-lateral retinotectal topographic mapping. Nature. 2006 Jan 5;439(7072) |
