Goat anti-Chat (mouse) Antibody

Item#:

dAP-0860

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Chat (mouse) Antibody
Principle Name	Chat (mouse)
Official Symbol	Chat
All Name and Symbols	Chat; choline acetyltransferase ; B230380D24Rik
Accession Number(s)	NP_034021.1
Peptide Sequence	KERARGPTKPSNLD, is from C Terminus
Human Gene ID(s)
Non-Human GeneID(s)	12647 (mouse); 432359 (rat);
Important Comments	This antibody is NOT expected to cross-react to Human CHAT.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 75-80kDa band observed in Mouse Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration
Immunohistochemistry	Frozen sections of Mouse Basal Forebrain shows staining of neural cell bodies. Recommended concentration
Reference(s)	Contestabile A, Fila T, Bartesaghi R, Contestabile A, Ciani E. Choline acetyltransferase activity at different ages in brain of Ts65Dn mice, an animal model for Down's syndrome and related neurodegenerative diseases. J Neurochem. 2006 Apr;97(2)