Goat anti-Prolyl Endopeptidase / PREP Antibody

Item#:

dAP-0856

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Prolyl Endopeptidase / PREP Antibody
Principle Name	Prolyl Endopeptidase / PREP
Official Symbol	PREP
All Name and Symbols	PREP; prolyl endopeptidase ; HGNC
Accession Number(s)	NP_002717.3
Peptide Sequence	QYIVGRSRKQSNP, is from internal region
Human Gene ID(s)	5550
Non-Human GeneID(s)	19072 (mouse); 83471 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe giv
Immunohistochemistry	In paraffin embedded Human Kidney shows distinct staining patterns in the cytoplam of PCT. Recommended concentration, 3-6µg/ml.
Reference(s)	Momeni N, Nordstrom BM, Horstmann V, Avarsaji H, Sivberg BV. Alterations of prolyl endopeptidase activity in the plasma of children with autistic spectrum disorders. BMC Psychiatry. 2005 Jun 2;5(1)