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Goat anti-Amphiphysin / AMPH Antibody
Item#:
dAP-0851
Size Price Quantity
50.0 ug $310.00
Goat anti-Amphiphysin / AMPH Antibody
Principle Name Amphiphysin / AMPH
Official Symbol AMPH
All Name and Symbols AMPH; amphiphysin; amphiphysin (Stiff-Man syndrome with breast cancer 128kDa autoantigen) ; HGNC
Accession Number(s) NP_001626.1; NP_647477.1
Peptide Sequence KRKDESRISKAE, is from internal region
Human Gene ID(s) 273
Non-Human GeneID(s) 218038 (mouse);
Important Comments This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 125kDa band observed in mouse brain lysates (calculated MW of 76.3kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997
Immunohistochemistry In paraffin embedded Human Cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4µg/ml.
Reference(s) Sommer C, Weishaupt A, Brinkhoff J, Biko L, Wessig C, Gold R, Toyka KV. Paraneoplastic stiff-person syndrome

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