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Goat anti-AIRE (isoform 1) Antibody
Item#:
dAP-0850
Size Price Quantity
50.0 ug $310.00
Goat anti-AIRE (isoform 1) Antibody
Principle Name AIRE (isoform 1)
Official Symbol AIRE
All Name and Symbols AIRE; autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) ; AIRE1; APECED; APS1; APSI; PGA1 ; autoimmune regulator (APECED protein); autoimmune regulator (automimmune polyendocrinopathy candidiasis ectodermal dystrophy);
Accession Number(s) NP_000374.1
Peptide Sequence KDVDLSQPRKGRKP, is from internal region
Human Gene ID(s) 326
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise isoform 1 (NP_000374.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Pig
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Appr. 60kDa in transfected HEK293 lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Thymus shows nuclear staining in select thymocytes. This product was confirmed in IHC on fetal Pig Thymus (PMID
Reference(s) Niki S, Oshikawa K, Mouri Y, Hirota F, Matsushima A, Yano M, Han H, Bando Y, Izumi K, Matsumoto M, Nakayama KI, Kuroda N, Matsumoto M. Alteration of intra-pancreatic target-organ specificity by abrogation of Aire in NOD mice. J Clin Invest. 2006 May 1;116(5)

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