Goat anti-EYA4 Antibody |
| Principle Name | EYA4 |
| Official Symbol | EYA4 |
| All Name and Symbols | EYA4; eyes absent homolog 4 (Drosophila); HGNC |
| Accession Number(s) | NP_004091.3; NP_742102.2; NP_742103.1 |
| Peptide Sequence | EDSQDLNEQSVKKTC, is from N Terminus |
| Human Gene ID(s) | 2070 |
| Non-Human GeneID(s) | 14051 (mouse); |
| Important Comments | This antibody is expected to recognise isoforms a, c and d (NP_004091.3, NP_742102.1 and NP_742103.1 respectively). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments gave an approx 50kDa band in Human and Mouse Brain lysates and in pituitary Mouse cell line NIH 3T3 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the |
| Immunohistochemistry | Paraffin embedded Human Testis and Liver. Recommended concentration |
| Reference(s) | Schonberger J, Wang L, Shin JT, Kim SD, Depreux FF, Zhu H, Zon L, Pizard A, Kim JB, Macrae CA, Mungall AJ, Seidman JG, Seidman CE. Mutation in the transcriptional coactivator EYA4 causes dilated cardiomyopathy and sensorineural hearing loss. Nat Genet. 2005 Feb 27; [Epub ahead of print] .PMID |
