Goat anti-AIF1 / IBA1 (isoform 3) Antibody

Item#:

dAP-0835

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-AIF1 / IBA1 (isoform 3) Antibody
Principle Name	AIF1 / IBA1 (isoform 3)
Official Symbol	AIF1
All Name and Symbols	AIF1; IBA1; allograft inflammatory factor 1 ; DASS-82G15.2; AIF-1; IRT-1; Em
Accession Number(s)	NP_001614.3
Peptide Sequence	NKQFLDDPKYSSDED, is from internal region
Human Gene ID(s)	199
Non-Human GeneID(s)	11629 (mouse);
Important Comments	This antibody is expected to recognise isoform 3 (NP_001614.3) only.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 17kDa band observed in Human Brain (Frontal Cortex) lysate (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration
Immunohistochemistry	In paraffin embedded Mouse Brain shows staining of astrocytes. Recommended concentration
Reference(s)	Yang ZF, Ho DW, Lau CK, Lam CT, Lum CT, Poon RT, Fan ST. Allograft inflammatory factor-1 (AIF-1) is crucial for the survival and pro-inflammatory activity of macrophages. Int Immunol. 2005 Nov;17(11)