Goat anti-Ku80 / XRCC5 Antibody

Item#:

dAP-0827

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Ku80 / XRCC5 Antibody
Principle Name	Ku80 / XRCC5
Official Symbol	XRCC5
All Name and Symbols	XRCC5; KU80; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining Ku autoantigen, 80kDa) ; HGNC
Accession Number(s)	NP_066964.1
Peptide Sequence	SLAKKDEKTDTLED, is from internal region
Human Gene ID(s)	7520
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 80-85kDa band observed in lysates of HeLa (calculated MW of 82.7kDa according to NP_066964.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Spleen. Recommended concentration
Reference(s)	Mayeur GL, Kung WJ, Martinez A, Izumiya C, Chen DJ, Kung HJ. Ku is a novel transcriptional recycling coactivator of the androgen receptor in prostate cancer cells. J Biol Chem. 2005 Mar 18;280(11)