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Goat anti-Hamartin / TSC1 (isoform 1) Antibody
Item#:
dAP-0823
Size Price Quantity
50.0 ug $310.00
Goat anti-Hamartin / TSC1 (isoform 1) Antibody
Principle Name Hamartin / TSC1 (isoform 1)
Official Symbol TSC1
All Name and Symbols TSC1; hamartin; tuberous sclerosis 1 ; HGNC
Accession Number(s) NP_000359.1
Peptide Sequence QLHIMDYNETHHEH, is from C Terminus
Human Gene ID(s) 7248
Non-Human GeneID(s) 64930 (mouse);
Important Comments This antibody is expected to recognise isoform 1 (NP_000359.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 150kDa band observed in Human Brain lysates (calculated MW of 130kDa according to NP_000359.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Miloloza et al Hum Mol Genet. 2000
Immunohistochemistry In paraffin embedded Human Uterus shows membranous staining around non-basal nuclei and at the lumen of the endometrial gland. Recommended concentration, 3-6µg/ml.
Reference(s) Chan JA, Zhang H, Roberts PS, Jozwiak S, Wieslawa G, Lewin-Kowalik J, Kotulska K, Kwiatkowski DJ. Pathogenesis of tuberous sclerosis subependymal giant cell astrocytomas

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