Goat anti-CYP3A4 Antibody

Item#:

dAP-0817

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CYP3A4 Antibody
Principle Name	CYP3A4
Official Symbol	CYP3A4
All Name and Symbols	CYP3A4; cytochrome P450, family 3, subfamily A, polypeptide 4 ; HGNC
Accession Number(s)	NP_059488.2; NP_001189784.1
Peptide Sequence	KESRLEDTQKHR, is from internal region
Human Gene ID(s)	1576
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_059488.2; NP_001189784.1) and it is not expected to cross-react with 3A5, 3A7 ot 3A43.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Preliminary experiments gave an approx 26-27kDa band in human liver lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57
Reference(s)	Choi MH, Skipper PL, Wishnok JS, Tannenbaum SR. Characterization of testosterone 11{beta}-hydroxylation catalyzed by human-liver microsomal cytochromes P450. Drug Metab Dispos. 2005 Mar 11; [Epub ahead of print] .PMID