Goat anti-HSD3B1 Antibody

Item#:

dAP-0813

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-HSD3B1 Antibody
Principle Name	HSD3B1
Official Symbol	HSD3B1
All Name and Symbols	HSD3B1; hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1; HGNC
Accession Number(s)	NP_000853.1
Peptide Sequence	DRHKETLKSKTQ, is from C Terminus
Human Gene ID(s)	3283
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 40kDa band observed in Human Placenta and Adrenal Gland lysates (calculated MW of 42.3kDa according to NP_000853.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Placenta. Recommended concentration
Reference(s)	Chang BL, Zheng SL, Hawkins GA, Isaacs SD, Wiley KE, Turner A, Carpten JD, Bleecker ER, Walsh PC, Trent JM, Meyers DA, Isaacs WB, Xu J. Joint effect of HSD3B1 and HSD3B2 genes is associated with hereditary and sporadic prostate cancer susceptibility. Cancer Res. 2002 Mar 15;62(6)