Goat anti-PDK1 Antibody |
| Principle Name | PDK1 |
| Official Symbol | PDK1 |
| All Name and Symbols | PDK1; pyruvate dehydrogenase kinase, isoenzyme 1; HGNC |
| Accession Number(s) | NP_002601.1 |
| Peptide Sequence | DFKDKSAEDAK, is from internal region |
| Human Gene ID(s) | 5163 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 65-70kDa band observed in Rat Heart lysates (calculated MW of 49.2kDa according to NP_002601.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended conce |
| Reference(s) | Sarbassov DD, Guertin DA, Ali SM, Sabatini DM. Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science. 2005 Feb 18;307(5712) |
