Goat anti-GLI2 Antibody |
| Principle Name | GLI2 |
| Official Symbol | GLI2 |
| All Name and Symbols | GLI2; GLI-Kruppel family member GLI2 ; THP2; oncogene GLI2; tax helper protein 2; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; zinc finger protein GLI2 |
| Accession Number(s) | NP_005261.2 |
| Peptide Sequence | DLKEDLDRDDCKQE, is from internal region |
| Human Gene ID(s) | 2736 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all four reported isoforms (NP_084655.1, NP_084656.1, NP_084657.1 and NP_005261.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Preliminary experiments gave an approx 55kDa band in Human Bone Marrow and Ovary lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated |
| Immunohistochemistry | In paraffin embedded Human Lymph Node shows nuclear staining in macrophages. Recommended concentration |
| Reference(s) | Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK. Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. PLoS Genet. 2005 Oct;1(4) |
