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Goat anti-P27KIP1 / CDKN1B (internal) Antibody
Item#:
dAP-0804
Size Price Quantity
50.0 ug $310.00
Goat anti-P27KIP1 / CDKN1B (internal) Antibody
Principle Name P27KIP1 / CDKN1B (internal)
Official Symbol CDKN1B
All Name and Symbols CDKN1B; KIP1; cyclin-dependent kinase inhibitor 1B (p27, Kip1) ; CDKN4; P27KIP1; cyclin-dependent kinase inhibitor 1B
Accession Number(s) NP_004055.1
Peptide Sequence KRPATDDSSTQNK, is from internal region
Human Gene ID(s) 1027
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2 (calculated MW of 22.1kDa according to NP_004055.1). The observed molecular weight corresponds to earlier findings in literature with different a
Immunohistochemistry In paraffin embedded Human Prostate shows nuclear staining of secretory cells. Recommended concentration, 5-10µg/ml.
Reference(s) Baldassarre G, Belletti B, Nicoloso MS, Schiappacassi M, Vecchione A, Spessotto P, Morrione A, Canzonieri V, Colombatti A. p27(Kip1)-stathmin interaction influences sarcoma cell migration and invasion. Cancer Cell. 2005 Jan;7(1)

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