Goat anti-GLI3 Antibody |
| Principle Name | GLI3 |
| Official Symbol | GLI3 |
| All Name and Symbols | GLI3; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) ; ACLS; GCPS; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV ; DNA-binding protein; GLI-Kruppel family member GLI3; oncogene GLI3; zinc finger protein GLI3 |
| Accession Number(s) | NP_000159.2 |
| Peptide Sequence | HNKRSKIKPDED, is from internal region |
| Human Gene ID(s) | 2737 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments in Human Brain, Lung, Placenta and Testes lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been |
| Reference(s) | Haycraft CJ, Banizs B, Aydin-Son Y, Zhang Q, Michaud EJ, Yoder BK. Gli2 and gli3 localize to cilia and require the intraflagellar transport protein polaris for processing and function. PLoS Genet. 2005 Oct;1(4) |
