Goat anti-CCKAR Antibody |
| Principle Name | CCKAR |
| Official Symbol | CCKAR |
| All Name and Symbols | CCKAR; cholecystokinin A receptor ; HGNC |
| Accession Number(s) | NP_000721.1 |
| Peptide Sequence | FEASQKKSAKERKPST, is from internal region |
| Human Gene ID(s) | 886 |
| Non-Human GeneID(s) | 12425 (mouse); 24889 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC, ICC, IF |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Western Blot | Preliminary experiments in Human Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). |
| Immunohistochemistry | In paraffin embedded Human Pancreas, shows cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration, 10µg/ml. |
| Reference(s) | Beglinger C, Degen L, Matzinger D, D'Amato M, Drewe J. Loxiglumide, a CCK-A receptor antagonist, stimulates calorie intake and hunger feelings in humans. Am J Physiol Regul Integr Comp Physiol. 2001 Apr;280(4) |
