Goat anti-Engrailed-2 Antibody

Item#:

dAP-0794

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Engrailed-2 Antibody
Principle Name	Engrailed-2
Official Symbol	EN2
All Name and Symbols	Engrailed-2; engrailed homolog 2 ; EN2; AUTS1
Accession Number(s)	NP_001418.2
Peptide Sequence	NHSTTAKEGKSDSE, is from C Terminus
Human Gene ID(s)	2020
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Mouse, Rat
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Approx. 30kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2kDa according to Human NP_001418.2 and of 33.8kDa according to Mouse NP_034264.1 and of 33.7kDa according to Rat NP_001102684.1). Recommended concentration
Reference(s)	Benayed R, Gharani N, Rossman I, Mancuso V, Lazar G, Kamdar S, Bruse SE, Tischfield S, Smith BJ, Zimmerman RA, Dicicco-Bloom E, Brzustowicz LM, Millonig JH. Support for the Homeobox Transcription Factor Gene ENGRAILED 2 as an Autism Spectrum Disorder Susceptibility Locus. Am J Hum Genet. 2005 Nov;77(5)