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Goat anti-SPEN / SHARP Antibody
Item#:
dAP-0793
Size Price Quantity
50.0 ug $310.00
Goat anti-SPEN / SHARP Antibody
Principle Name SPEN / SHARP
Official Symbol SPEN
All Name and Symbols SPEN; spen homolog, transcriptional regulator (Drosophila) ; KIAA0929; MINT; RP1-134O19.1; SHARP; Msx2 interacting nuclear target (MINT) homolog; OTTHUMP00000044600; SMART/HDAC1 associated repressor protein; nuclear receptor transcription cofactor spen ho
Accession Number(s) NP_055816.2
Peptide Sequence DSEGKMDDKKEDHKE, is from internal region
Human Gene ID(s) 23013
Non-Human GeneID(s)
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of approx. 402kDa according to NP_055816.2. Therefore we cannot recommend an optimal concentration and the pr
Reference(s) Hiriart E, Gruffat H, Buisson M, Mikaelian I, Keppler S, Meresse P, Mercher T, Bernard OA, Sergeant A, Manet E. Interaction of the Epstein-Barr virus mRNA export factor EB2 with human Spen proteins SHARP, OTT1, and a novel member of the family, OTT3, links Spen proteins with splicing regulation and mRNA export. J Biol Chem. 2005 Nov 4;280(44)

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