Goat anti-CDKN2A (isoform 1) / p16INK4a Antibody |
| Principle Name | CDKN2A (isoform 1) / p16INK4a |
| Official Symbol | CDKN2A |
| All Name and Symbols | CDKN2A; p16INK4a; cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) ; HGNC |
| Accession Number(s) | NP_000068.1; NP_001182061.1 |
| Peptide Sequence | HARIDAAEGPSDIPD, is from C Terminus |
| Human Gene ID(s) | 1029 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise isoform p16INK4a (NP_000068.1) and isoform p16gamma (NP_001182061.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx. 16-18kDa band observed in lysates of the cell lines A431 and HeLa (calculated MW of 16.5kDa according to NP_000068.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Liver. Recommended concentration |
| Reference(s) | Mawrin C, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk CK, von Mawrin A, Kirches E, Schneider-Stock R, Boltze C, Vorwerk Alterations of cell cycle regulators in gliomatosis cerebri. J Neurooncol. 2005 Apr;72(2) |
