Goat anti-PCK1 / PEPCKC (internal) Antibody

Item#:

dAP-0782

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PCK1 / PEPCKC (internal) Antibody
Principle Name	PCK1 / PEPCKC (internal)
Official Symbol	PCK1
All Name and Symbols	PCK1; phosphoenolpyruvate carboxykinase 1 (soluble); HGNC
Accession Number(s)	NP_002582.3
Peptide Sequence	HVNWFRKDKEGK, is from internal region
Human Gene ID(s)	5105
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Preliminary experiments gave bands at approx 70kDa, 45kDa and 28kDa in Human Adipose, Kidney and Liver lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the additional
Immunohistochemistry	In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in pyramidal cells. Recommended concentration, 2-4µg/ml.
Reference(s)	Yang Q, Graham TE, Mody N, Preitner F, Peroni OD, Zabolotny JM, Kotani K, Quadro L, Kahn BB. Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes. Nature. 2005 Jul 21;436(7049)