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Goat anti-TRIM5 / RNF88 (alpha isoform) Antibody


Item#:
dAP-0772
Size Price Quantity
50.0 ug $310.00
Goat anti-TRIM5 / RNF88 (alpha isoform) Antibody
Principle Name TRIM5 / RNF88 (alpha isoform)
Official Symbol TRIM5
All Name and Symbols TRIM5; tripartite motif-containing 5 ; HGNC
Accession Number(s) NP_149023.1
Peptide Sequence CNIEKNENYQPK, is from internal region
Human Gene ID(s) 85363
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise isoform alpha (NP_149023.1) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 37kDa band in Lysates of Human cell lines Daudi, Jurkat and MOLT4 after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe g
Immunohistochemistry In paraffin embedded Human Kidney shows cytoplasm staining in PCT. Recommended concentration, 3-6µg/ml.
Reference(s) Berthoux L, Sebastian S, Sokolskaja E, Luban J. Cyclophilin A is required for TRIM5{alpha}-mediated resistance to HIV-1 in Old World monkey cells. Proc Natl Acad Sci U S A. 2005 Oct 11;102(41)

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