Goat anti-Stearoyl-CoA desaturase Antibody

Item#:

dAP-0770

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Stearoyl-CoA desaturase Antibody
Principle Name	Stearoyl-CoA desaturase
Official Symbol	SCD
All Name and Symbols	SCD; SCD1; HGNC
Accession Number(s)	NP_005054.3
Peptide Sequence	RIKRTGDGNYKSG, is from C Terminus
Human Gene ID(s)	6319
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC, FC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Preliminary experiments gave an approx 30kDa band in Human Adipose, Brain and Liver lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc
Immunohistochemistry	In paraffin embedded Human Liver shows textured cytoplam staining in hepatocytes. Recommended concentration, 5-10µg/ml.
Reference(s)	Zhang S, Yang Y, Shi Y. Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells. Biochem J. 2004 Dec 20; [Epub ahead of print] .PMID