Goat anti-14-3-3 sigma / Stratifin Antibody

Item#:

dAP-0766

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-14-3-3 sigma / Stratifin Antibody
Principle Name	14-3-3 sigma / Stratifin
Official Symbol	SFN
All Name and Symbols	SFN; 14-3-3sigma; stratifin; HGNC
Accession Number(s)	NP_006133.1
Peptide Sequence	DLHTLSEDSYKDST, is from internal region
Human Gene ID(s)	2810
Non-Human GeneID(s)	55948 (mouse); 313017 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx. 28kDa band observed in Human Skin lysates (calculated MW of 27.8kDa according to NP_006133.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Skin shows strong nuclear staining in selected cells of the basal layer. Recommended concentration, 2-4µg/ml.
Reference(s)	Lodygin D, Hermeking H. The role of epigenetic inactivation of 14-3-3sigma in human cancer. Cell Res. 2005 Apr;15(4)