Goat anti-PDE4D Antibody |
| Principle Name | PDE4D |
| Official Symbol | PDE4D |
| All Name and Symbols | PDE4D; phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila); HGNC |
| Accession Number(s) | NP_001184151.1; NP_001184150.1; NP_006194.2; NP_001098101.1; NP_001184152.1; NP_001159371.1 |
| Peptide Sequence | QPEACVIDDRSPDT, is from C Terminus |
| Human Gene ID(s) | 5144 |
| Non-Human GeneID(s) | |
| Important Comments | This antiobody is expected to recognize all reported isoforms. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB Trf, IHC, EIA |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 70+80+90kDa bands observed in lysates of cell line A549. In transfected COS1 transiently expressing PDE4D1 and PDE4D3 bands of approx. 68kDa and 95kDa are observed. These bands are not observed in the untransfected COS1. Calculated |
| Immunohistochemistry | Paraffin embedded Human Skeletal Muscle and Heart. Recommended concentration |
| Reference(s) | Houslay MD. The long and short of vascular smooth muscle phosphodiesterase-4 as a putative therapeutic target. Mol Pharmacol. 2005 Sep;68(3) |
