Goat anti-VPS25 Antibody |
| Principle Name | VPS25 |
| Official Symbol | VPS25 |
| All Name and Symbols | VPS25; vacuolar protein sorting 25 (yeast); HGNC |
| Accession Number(s) | NP_115729.1 |
| Peptide Sequence | QPNVDTRQKQ, is from internal region |
| Human Gene ID(s) | 84313 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx. 22kDa band observed in Rat Kidney lysates (calculated MW of 20.7kDa according to human NP_115729.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded human kidney, shows granular cytoplasmic staining of endothelial cells. Data obtained from the previous batch (different goat). Recommended concentration, 10µg/ml. |
| Reference(s) | Vaccari T, Bilder D. The Drosophila Tumor Suppressor vps25 Prevents Nonautonomous Overproliferation by Regulating Notch Trafficking. Dev Cell. 2005 Nov;9(5) |
