Goat anti-MURF1 / TRIM63 (internal) Antibody

Item#:

dAP-0752

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MURF1 / TRIM63 (internal) Antibody
Principle Name	MURF1 / TRIM63 (internal)
Official Symbol	TRIM63
All Name and Symbols	TRIM63; tripartite motif-containing 63 ; HGNC
Accession Number(s)	NP_115977.2
Peptide Sequence	QQYQEQLDKSTKLVE, is from internal region
Human Gene ID(s)	84676
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave an approx 75kDa band in Human Heart and Skeletal Muscle lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc
Immunohistochemistry	Paraffin embedded Human Skeletal Muscle. Recommended concentration
Reference(s)	Cao PR, Kim HJ, Lecker SH. Ubiquitin-protein ligases in muscle wasting. Int J Biochem Cell Biol. 2005 Oct;37(10)